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. 2021 Sep 10;11:748795. doi: 10.3389/fcimb.2021.748795

Figure 2.

Figure 2

Overexpression and knockdown of SOCS3 in DF-1 cells could influence ALV-J virus replication. After 24 h of overexpression of SOCS3 in DF-1 cells, cells were infected with 105 TCID50/mL ALV-J strain SCAU-HN06. Samples were collected at 24 and 48 hpi, and qRT-PCR (A), ELISA (B), and WB (C) were performed for ALV-J virus content. After 24 h knockdown of SOCS3, DF-1 cells were infected with 105 TCID50/mL ALV-J strain SCAU-HN06. Samples were collected at 24 and 48 hpi, and qRT-PCR (D), ELISA (E), and WB (F) were performed for ALV-J virus content. These experiments were performed at least three times independently with similar results. Differences in data were evaluated by the Student’s t-test t test. The error bars are the standard error of the mean (SEMs) (***P ≤ 0.01).