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. 2021 Sep 10;11:748795. doi: 10.3389/fcimb.2021.748795

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Copyright © 2021 Mo, Fu, Hu, Zhang, Xian, Zhang, Lin, Shi, Nie and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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JAK2 and STAT3 gene expression in tissues and cells. The (A) JAK2 and (B) STAT3 expression in immune organs of ALV-J infected chickens (n = 3) and uninfected chickens (n = 3) were quantified by qRT-PCR. The chickens were only infected with ALV-J, as previously demonstrated by Zhang et al. (2021). After DF-1 cells were infected with ALV-J strain SCAU-HN06, the (C) JAK2 and (D) STAT3 genes expression was detected by qRT-PCR from 12 hpi to 96 hpi. These experiments were performed independently at least three times with similar results. Differences in data were evaluated by the Student’s t-test. The error bars are the standard error of the mean (SEMs) (*P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001).