Figure 7.

Overexpression of SOCS3 promotes the expression of interferons, inflammatory factors, and interferon-stimulated genes. After SOCS3 overexpression in DF-1 cells in 24h, the cells were infected with 10⁵ TCID₅₀/mL ALV-J strain SCAU-HN06. At 24 and 48 hpi, qRT-PCR was used to detect (A) IFNα, (B) IFNβ, (C) IL-6, (D) TNFα, (E) CH25H, (F) MX1, (G) OASL, and (H) ZAP mRNA levels. After three freeze-thaw cycles, the levels of the content of (I) IFNα, (J) IFNβ, (K) IL-6, and (L) TNFα in cells were detected by ELISA assay. These experiments were performed independently at least three times with similar results. Differences in data were evaluated by the Student’s t-test. The error bars are the standard error of the mean (SEMs) (*P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001).