Figure 3.

Antitumor efficacy of 806 CAR T cells in primary astrocytes and keratinocytes. (A) Surface expression of EGFR assessed by flow cytometry on human primary astrocytes and keratinocytes using EGFR-specific cetuximab antibody. (B) Primary astrocytes and keratinocytes were cocultured with 806 CAR T cells at indicated ratios in a 4-h chromium assay, and results are representative of a single experiment showing the average ± SD of triplicates. (C) Levels of IFN-γ measured in supernatants by ELISA 24 h after coculturing 806 and 2173 CAR T cells with primary astrocytes and keratinocytes at an effector-to-target ratio of 1:1. Results are representative of a single experiment with the average ± SD of triplicates.