FIG. 5.

ASH1 and TRX SET domains interact in vitro. Interaction was analyzed by GST pull-down assays (A), coimmunoprecipitation (IP) of in vitro-produced polypeptides (B), and coimmunoprecipitation of polypeptides expressed in transfected cells (C). The radiolabeled TRX SET polypeptide in panels A and B and the epitope-tagged polypeptides in panel B were made by coupled transcription-translation. GST-ASH1 SET and GST polypeptides in panel A were produced in bacteria. In panel B, p62 related and Dsup35 are Drosophila proteins, which served in this experiment as controls for specificity. HA (hemagglutinin) and T7 are epitope tags. The positions of the molecular size markers are indicated on the left.