FIG. 3.

Apo-RXR exists as an intermediate between CoR- and CoA-interacting conformations. (A) Apo-RXR and apo-TR are differentially sensitive to proteolysis. Arrows point to protected fragments. Proteins were translated in reticulocyte lysate and treated with trypsin (300 μg/ml) for various times (5, 10, and 20 min). The T3 concentration was 1 μM and SR11237 was used at a concentration of 50 μM. (B) Differential protease sensitivity of apo-RXRΔ443 and RXR-F313A.