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. 2021 Sep 13;22(5):1282. doi: 10.3892/etm.2021.10717

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Copyright: © Garcia-Ruiz et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Expression of mesenchymal and chondrogenic markers in Ad-MSCs determined by reverse transcription-quantitative PCR. Ad-MSCs subjected to the differentiation schemes A-E were analyzed for (A) mesenchymal marker [THY1 (CD90)] and (B) chondrogenic marker (SOX9, ACAN, COMP and COL2A1) expression at different time points of exposure. Dotted lines indicate the change from monolayer to biphasic scaffold. ^*P<0.05 (group B vs. all other groups). Ad-MSCs, adipose-derived mesenchymal stem cells; THY1, Thy-1 cell surface antigen; ACAN, aggrecan; COMP, cartilage oligomeric matrix protein; COL2A1, collagen type II α 1 chain.