Table I.
PCR primers designed for mesenchymal, chondrogenic and osteogenic markers.
| A, Chondrogenic markers | ||
|---|---|---|
| Gene | Forward primer (5'-3') | Reverse primer (5'-3') |
| SOX9 | AACGGCTCCAGCAAGAACAAG | GCTCCGCCTCCTCCACGAAG |
| ACAN | CAACAATGCCCAAGACTACCAG | TTCCACTCGCCCTTCTCGTG |
| COMP | CAGACAATGAACAGCGACCC | GCCTGCCAATACGTTTGCTC |
| COL2A1 | TCATCCAGGGCTCCAATGACGTG | AACAGTCTTGCCCCACTTACCG |
| B, Osteogenic markers | ||
| Gene | Forward primer (5'-3') | Reverse primer (5'-3') |
| RUNX2 | GAACTCGTCCGCACCGACAG | ATCGTTACCCGCCATGACAGT |
| ALPL | CGGCCTGGACCTCGTTGACA | ACGTTGTTCCTGTTCAGCTCGTA |
| MMP13 | CGCCAGACAAATGTGACCCTT | AAAACAGCTCCGCATCAACC |
| COL10A1 | CGCCAGACAAATGTGACCCTT | AAAACAGCTCCGCATCAACC |
| COL1A2 | TAGAAAGAACCCAGCTCGCACA | GGTTTCGCCAGTAGAGAAATCACA |
| SPP1 | AAGAAGTTTCGCAGACCTGACATCC | TGCACCATTCAACTCCTCGCTTT |
| C, Mesenchymal marker | ||
| Gene | Forward primer (5'-3') | Reverse primer (5'-3') |
| THY1 | CACACATACCGCTCCCGAAC | CTGATGCCCTCACACTTGACCA |
| D, Endogenous gene | ||
| Gene | Forward primer (5'-3') | Reverse primer (5'-3') |
| GAPDH | ACAACAGCCTCAAGATCATCAGC | TCACGCCACAGTTTCCCGGAG |
THY1, Thy-1 cell surface antigen; ACAN, aggrecan; COMP, cartilage oligomeric matrix protein; COL2A1, collagen type II α 1 chain; RUNX2, runt-related transcription factor 2; ALPL, alkaline phosphatase; SPP1, osteopontin.