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. 2021 Mar 19;121(18):11030–11084. doi: 10.1021/acs.chemrev.0c01074

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© 2021 The Authors. Published by American Chemical Society

Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).

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Solution-based modification of ODNs for functional handle attachment. (A)–(D) The attachment of RAFT agents, (((butylthio)carbonothioyl)thio)propanoic acid (BTPA) and 4-cyano-4-(phenylcarbonothioylthio)pentanoic acid (CPADB), to ODNs through amide coupling chemistry with NHS and pentafluorophenol (PFP) activated carboxylic acids. (E) NHS-activated coupling of tetrazine to amine DNA. (F) Amide coupling of norbornene-carboxylic acid with amine DNA. Coupling reagents include diisopropylethylamine (DIPEA), 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDCl), and 1-hydroxybenzotriazole (HOBt), and solvents include dimethylformamide (DMF) and phosphate buffered saline (PBS).