Fig. 2.

Aβ plaque load in App^NLF KI mice is not altered by neuronal cell cycle re-entry. A) DAB stained Immunohistochemical (6E10 antibody) image showing Aβ deposition in the primary somatosensory areas of cortex in App^NLF KI and NCCR-App^NLF KI mice. Scale bar 100μm. B) Representative immunofluorescence images of C-terminal Aβ₄₂ antibody-positive Aβ plaque staining in the primary somatosensory areas of cortex in App^NLF and NCCR-App^NLF mice at 9, 12, and 18 months of age used for Aβ plaque load analysis. Insert is a magnified image of the region inside the white square. Cortical areas covered by C-terminal Aβ₄₂ positive extracellular signals were quantified in four matched coronal brain sections from each perfusion fixed animals using the Area Fraction Fractionator probe (Stereoinvestigator, MBF). Scale bar 200μm. C) Two-way ANOVA showed significant age effect (F (2, 140)  = 3437, p < 0.0001) but no difference between genotypes (F (1, 140) = 3.637, p = 0.0586), 9 months: n = 8/group; 12 months: n = 8-9/group; 18 months: n = 2/group. Bars represent mean±SEM.