Fig. 4.

Neuronal cell cycle re-entry increases astrocytosis in App^NLF mice. A) Representative fluorescently labeled images of cortical areas (primary somatosensory region) stained using GFAP antibody. B) Magnification of white inset in (A) demonstrating astrocyte specific GFAP-labeling. C) Grayscale images of GFAP immunofluorescence labeled whole cortical regions in brain sections from 9‐, 12‐, and 18‐month-old NCCR‐App^NLF and App^NLF animals were evaluated using Image J. D) Two-way ANOVA shows significant age effect (F(2, 105) = 196, p < 0.0001), genotype effect (F(1, 105) = 359.3, p < 0.0001), and interaction effect (F(2, 105) = 24.68, p < 0.0001). GFAP immunoreactive cortical area is greater in the NCCR- App^NLF animals compared to age-matched App^NLF animals at all ages. Furthermore, NCCR-App^NLF animals show progressive increase with age (Tukey’s multiple comparison test, 9 months versus 12 months, p < 0.0001; 9 months versus 18 months, p < 0.0001; 12 months versus 18 months, p < 0.0001). Following numbers of animals were examined; at 9 months of age: NCCR-App^NLF, n = 4; App^NLF, n = 4‐ at 12 months of age: NCCR-App^NLF, n = 4; App^NLF, n = 4; at 18 months of age: NCCR-App^NLF, n = 2; App^NLF, n = 2. Four matched coronal sections were measured per animal. Each data point represents a measure from each section.