Fig. 1.
Loss of noradrenergic afferents is observed in cortical and hippocampal regions in 12- and 24-month-old AppNL-G-F/NL-G-F mice. A) Representative images of the neocortex and hippocampal CA1 subfield from frozen coronal brain sections immunostained with anti-NET (indicated by green) were shown (blue indicated DAPI staining). B, C) Total length of NET-positive fiber was measured and expressed as fiber length per area (μm/μm2) in the neocortex (B) and hippocampal CA1 subfield (C). D) Representative images of the prefrontal and entorhinal cortices from frozen coronal brain sections immunostained with anti-NET (indicated by green) were shown (blue indicated DAPI staining). E, F) Total length of NET-positive fiber was measured and expressed as fiber length per area (μm/μm2) in the prefrontal cortex (E) and entorhinal cortex (F). Scale bars represent 50μm. In the inset image, scale bar represents 10μm. n = 4–5/group. ***p < 0.001 versus WT (B6J); †††p<0.001 versus AppNL/NL; §§p < 0.01 versus 12-month-old AppNL-G-F/NL-G-F. n.s., not significant.