Fig. 4.

Noradrenergic axonal degeneration is not limited to the vicinity of Aβ plaques and reactive gliosis. A, B) Representative images of the neocortex (A) and hippocampal CA1 subfield (B) from frozen coronal brain sections immunostained with anti-NET (indicated by green in A and B), anti-Iba1 (indicated by magenta in upper panels of A and B) and anti-GFAP (indicated by magenta in lower panels of A and B) antibodies were shown. FSB was used for detecting Aβ plaques (indicated by blue in A and B). White arrows point to representative examples of aberrant NET-positive fiber cluster. Insets show higher-magnification views in the corresponding dashed yellow squares. Scale bars represent 50μm. In the inset images, scale bars represent 10μm. n = 3/group.