Table 1.
Breakdown of statistics and significant effects and/or interactions for all measures
| Data | Type of Test | Factors | Significant Finding(s) | t or F statistic | p | Corrections Applied |
| Fig. 2D aSyn and pSyn Protein | 2-way ANOVA | PFF Status (PFF/Mono) | Main effect of ASO (asyn) | F(1,29) = 74.33 | p < 0.0001 | Sidak’s |
| ASO Staus (ASO/Scramble) | Main effect of ASO (psyn) | F(1,29) = 35.88 | p < 0.0001 | Sidak’s | ||
| Fig. 2E pSyn Inclusions: Composite Score | Independent Samples t-test | ASO Status (ASO/Scramble) | Main effect of ASO | t(65) = 2.280 | p = 0.0259 | |
| Fig. 2E pSyn Inclusions: Motor Cortex | Independent Samples t-test | ASO Status (ASO/Scramble) | No effects | t(15) = 1.117 | p = 0.281 | |
| Fig. 2E pSyn Inclusions: Hippocampus | Independent Samples t-test | ASO Status (ASO/Scramble) | No effects | t(14) = 1.067 | p = 0.304 | |
| Fig. 2E pSyn Inclusions: Medial Somatosensory Cortex | Independent Samples t-test | ASO Status (ASO/Scramble) | No effects | t(15) = 1.145 | p = 0.270 | |
| Fig. 2E pSyn Inclusions: Lateral Somatosensory Cortex | Independent Samples t-test | ASO Status (ASO/Scramble) | No effects | t(15) = 1.022 | p = 0.23 | |
| Fig. 3B-C Water Maze Cumulative Distance during Hidden Platform Location | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Time x PFF | F(8.472,245.702) = 2.025 | p = 0.041 | Greenhouse-Geisser |
| ASO Status (ASO/Scramble) Time | Main effect of PFF | F(1,29) = 6.638 | p = 0.015 | |||
| Time x ASO | F(8.472,245.702) = 2.626 | p = 0.008 | Greenhouse-Geisser | |||
| Fig. 3B-C Water Maze Cumulative Distance during Visible Platform Location | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Time x ASO | F(7.527,218.278) = 3.705 | p = 0.001 | Greenhouse-Geisser |
| ASO Status (ASO/Scramble) Time | ||||||
| Fig. 3E Water Maze Change in Cumulative Distance to Hidden Platform Locations | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Main effect of PFF | F(1,29) = 4.159 | p = 0.05 | |
| ASO Status (ASO/Scramble) Time | ||||||
| Fig. 3E Water Maze Change in Cumulative Distance to Visible Platform Locations | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Main effect of ASO | F(1,29) = 7.049 | p = 0.012 | |
| ASO Status (ASO/Scramble) Time | ||||||
| Fig. 3F Water MazeCumulative Distance during Probe Trials | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Time x PFF | F(4.283,124.217) = 3.257 | p = 0.005 | Greenhouse-Geisser |
| ASO Status (ASO/Scramble) Time | Main effect of PFF | F(1,29) = 9.372 | p = 0.012 | Greenhouse-Geisser | ||
| Fig. 3G Water Maze Percent Time in Target Quadrant during Probe Trials | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Time x PFF | F(7,203) = 3.353 | p = 0.001 | |
| ASO Status (ASO/Scramble) Time | Main effect of PFF | F(1,29) = 8.043 | p = 0.008 | |||
| Time x ASO | F(7,203) = 2.083 | p = 0.047 | ||||
| Fig. 4B Total Distance Moved in the Open Field | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Time x PFF x ASO | F(1,29) = 5.637 | p = 0.024 | |
| ASO Status (ASO/Scramble) Time | ||||||
| Average Velocity in the Open Field | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Time x PFF x ASO | F(1,29) = 5.533 | p = 0.026 | |
| ASO Status (ASO/Scramble) Time | ||||||
| Fig. 4C Novel Object 3 mpi | Paired Samples t-test | Object (Familiar/Novel) | Mono-Scramble | t(50) = 5.632 | p < 0.0001 | Sidak’s |
| Mono-ASO | t(50) = 4.706 | p < 0.0001 | Sidak’s | |||
| PFF-Scramble | t(50) = 6.796 | p < 0.0001 | Sidak’s | |||
| PFF-ASO | t(50) = 6.713 | p < 0.0001 | Sidak’s | |||
| Fig. 4D Novel Object 6 mpi | Paired Samples t-test | Object (Familiar/Novel) | Mono-Scramble | t(52) = 4.206 | p = 0.0004 | Sidak’s |
| Mono-ASO | t(52) = 1.845 | p = 0.2541 | Sidak’s | |||
| PFF-Scramble | t(52) = 1.945 | p = 0.2099 | Sidak’s | |||
| PFF-ASO | t(52) = 3.462 | p = 0.0043 | Sidak’s | |||
| Fig. 5B Latency to Fall off the Rotarod | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Time x ASO | F(3,87) = 16.145 | p < 0.0001 | |
| ASO Status (ASO/Scramble) Time | ||||||
| Fig. 5C Wire Hang Latency to Fall | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Main effect of ASO | F(1,26) = 21.388 | p < 0.001 | |
| ASO Status (ASO/Scramble) Time | Time x ASO | F(1,26) = 11.936 | p = 0.002 | |||
| PFF x ASO | F(1,26) = 6.030 | p = 0.021 | ||||
| Fig. 5C Wire Hang Fall Score | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Main effect of ASO | F(1,26) = 13.826 | p = 0.001 | |
| ASO Status (ASO/Scramble) Time | Time x ASO | F(1,26) = 12.923 | p = 0.002 | |||
| Trend towards main effect of PFF | F(1,26) = 3.739 | p = 0.064 | ||||
| Fig. 5E Wire Hang Fall Score over Time 3 mpi | Repeated Measures ANOVA | PFF Status (PFF/Mono) | No effects | |||
| ASO Status (ASO/Scramble) Time | ||||||
| Fig. 5F Wire Hang Fall Score over Time 6 mpi | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Main effect of ASO | F(1,26) = 20.089 | p < 0.001 | |
| ASO Status (ASO/Scramble) Time | Time x ASO | F(35,910) = 6.087 | p < 0.001 | |||
| Time x PFF | F(35,910) = 3.409 | p < 0.001 | ||||
| Fig. 5G Wire Hang Latency to Reach | Repeated Measures ANOVA | PFF Status (PFF/Mono) | No effects | |||
| ASO Status (ASO/Scramble) Time | ||||||
| Fig. 5H Wire Hang Reach Score | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Trend towards main effect of PFF | F(1,26) = 3.250 | p = 0.084 | |
| ASO Status (ASO/Scramble) Time | ||||||
| Fig. 5I Wire Hang Reach Score over Time 3 mpi | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Time x PFF | F(35,980) = 3.195 | p < 0.001 | |
| ASO Status (ASO/Scramble) Time | ||||||
| Fig. 5J Wire Hang Reach Score over Time 6 mpi | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Time x ASO | F(35,910) = 5.116 | p < 0.001 | |
| ASO Status (ASO/Scramble) Time | Time x PFF x ASO | F(35,910) = 4.036 | p < 0.001 | |||
| PFF x ASO | F(1,26) = 6.770 | p = 0.015 | ||||
| Trend towards main effect of ASO | F(1,26) = 3.995 | p = 0.056 | ||||
| Fig. 6A Average Daytime Activity | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Time x ASO | F(1.703,34.060) = 3.550 | p = 0.046 | Huynh-Feldt |
| ASO Status (ASO/Scramble) Time | ||||||
| Fig. 6B Average Nighttime Activity | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Trend towards a Time x ASO interaction | F(3,60) = 2.316 | p = 0.085 | |
| ASO Status (ASO/Scramble) Time | ||||||
| Fig. 6C Body Weight | Repeated Measures ANOVA | PFF Status (PFF/Mono) | Time x ASO | F(3.613,104.777) = 16.093 | p < 0.001 | Huynh-Feldt |
| ASO Status (ASO/Scramble) Time | Main effect of ASO | F(1,29) = 7.952 | p < 0.001 | |||
| Fig. 6D Food Intake | 2-way ANOVA | PFF Status (PFF/Mono) | No effects (Daytime) | |||
| ASO Staus (ASO/Scramble) | Main effect of ASO (Nighttime) | F(1,26) = 5.297 | p = 0.0296 | |||
| Fig. 6F Body Weight in aSyn KO mice | Repeated Measures ANOVA | ASO Status (ASO/Scramble) Time | Time x ASO | F(16,88) = 6.079 | p = 0.0001 | |
| Main effect of ASO | F(2,11) = 19.03 | p = 0.0003 |