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Mast cell activators increase B220+ cells in the cervical lymph nodes after nasal exposure. C57BL/6 mice were nasally instilled with saline, M7, ST101036, R127655, R529877, ST027688, or ST048871. After 24 hours, cervical lymph nodes (cLN) were obtained. Single-cell suspension of isolated cLNs was analyzed by flow cytometry (A). One-way ANOVA determined a significant increase compared to saline **p < 0.01, ***p < 0.001. Bars represent the mean + SEM (B).
