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. 2021 Sep 13;7(9):1581–1590. doi: 10.1021/acscentsci.1c00811

Figure 4.

Figure 4

Esterase-catalyzed hydrolysis of abscisic acid (ABA) can support phage replication. (a) BS2 activity on ABA esters. ABA esters (750 μM) were incubated with 5 μM BS2 over 60 min, and percent conversion to ABA was determined. (b) BS2 phage replication with ABA derivatives. Phage carrying BS2 were incubated with E. coli expressing ABA biosensor in the absence or presence of ABA esters or ABA for 6 h. Phage cultures were then collected and analyzed for phage replication. Error bars are the standard error for n = 3 replicates.