Skip to main content
. 2021 Sep 13;7(9):1581–1590. doi: 10.1021/acscentsci.1c00811

Figure 6.

Figure 6

Evaluation of substrate scope for selected hits from a PACS screen. (a) Reaction scheme for ABA esters evaluated. 750 μM substrate was incubated with 5 μM BS2 variant for 1 h. (b) Conversion of each reaction normalized to the WT reaction. Each bar represents the average of three reactions. Yield of ABA obtained from a calibration curve using 2-acetamidophenol as an internal standard (calibration curve Figure S17). (c) Reaction scheme for the hydrolysis of “truncated” ABA fragment, 3,3-dimethyl acrylate (DMA) esters. 5 mM substrate was incubated with 10 μM BS2 variant for 1 h. (d) Conversion of each reaction normalized to the WT reaction. Each bar represents the average of three reactions. Yield of 3,3-DMA obtained from a calibration curve using 5-bromoindole as the internal standard (calibration curve Figure S18).