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. 2021 Jul 28;321(3):C519–C534. doi: 10.1152/ajpcell.00156.2021

Figure 5.

Figure 5.

SLC25A3 deletion decreases levels of sirtuin 3 (SIRT3) in mitochondria. A: cardiac acetyl-CoA levels in Slc25a3fl/fl vs. Slc25a3fl/flxMCM mice at 10 wk posttamoxifen administration (n = 6 fl/fl and n = 6 fl/flxMCM animals). One-way ANOVA followed by Welch’s test was used for statistical analysis, with P < 0.05 considered significant. *P < 0.05 B: Western blot analysis of GCN5L1 expression in total heart protein lysates from Slc25a3fl/fl vs. Slc25a3fl/flxMCM animals. GAPDH was used as a loading control. C: quantification of cardiac GCN5L1 levels in the indicated groups (n = 3 animals/group). Student’s t test was used for statistical analysis, with P < 0.05 considered significant. *P < 0.05. D: Western blot analysis of SIRT3 expression in cardiac mitochondria isolated from Slc25a3fl/fl vs. Slc25a3fl/flxMCM animals. Voltage dependent anion channel (VDAC) was used as a mitochondrial loading control. E: quantification of mitochondrial SIRT3 levels in cardiac mitochondria from the indicated groups (n = 3 animals/group). Student’s t test was used for statistical analysis with P < 0.05 considered significant. *P < 0.05.