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. 2021 Sep 23;9:33. doi: 10.1186/s40170-021-00270-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — PFK158 reduces tumor growth in a MYC^High xenograft model. A H446 xenografts were given 25 mg/kg PFK158 by intraperitoneal injection every other day for 10 days. B PFK158-treated animals exhibited a significant delay in tumor growth. C and D H&E staining showing that PFK158-treated animals have less necrotic tissue. E Immunoblot against MYC and PFKFB3 with loading control Vinculin. F PFKFB3 expression was significantly reduced in H446 tumors from animals treated with PFK158. G and H MYC expression was lower but not significantly so in tumors regardless of PFK158 treatment when evaluated by immunoblotting or flow cytometry, respectively. I Heatmap showing transcriptional expression changes between H446 tumors treated with vehicle or PFK158. J–L SLC2A1, HK2, and PFKFB3 gene expression are not significantly altered following PFK158 treatment. M–O Downstream glycolysis enzyme genes ALDOA, ENO1, and LDHA are significantly lower following PFK158 treatment. (*P < 0.05; ***P < 0.005)