Fig. 7.

Blocking Nrf2 and autophagy abrogated the protective effects of calcitriol (0.5 µg/kg) on TBI-induced neurological dysfunction. a The variation in neurological deficits at 14 days after treatment was determined by neurological severity score tests. b The time (seconds) spent finding the submerged platform at 14 days. c The time (seconds) spent exploring the quadrant that initially contained the platform at 14 days. d Representative traces from the probe trials indicating the sample paths of the mice. e Representative traces after the platform was removed indicating the sample paths of the mice. f Statistical graphs of apoptotic cells (% of DAPI). g Representative immunofluorescence assays of TUNEL-positive cells (Bar = 50 µm). Data are presented as means ± SEM (n = 10). *P < 0.05 and **P < 0.01 versus the indicated groups. (TBI: TBI model group; Calcitriol: TBI + calcitriol treatment group; CQ: TBI + calcitriol + CQ treatment group; Nrf2^−/−: TBI + calcitriol + Nrf2 knockout group)