Fig. 5.

CMTM3 induces changes in gene expression profiles. A The number of different expressed genes (padj < 0.05, MOI: 100). B A heat map summary of the gene expression values of JHC7-MOCK and JHC7-CMTM3 cells. Red indicates high and green indicates low gene expression values (padj < 0.001). C KEGG enrichment analysis. The TOP seven upregulated signaling pathways are shown of CMTM3-vs-MOCK in JHC7 cells (padj < 0.05). D The expression of TP53 was detected in CMTM3 overexpressed chordoma cell lines by Real-time PCR. E The expression of TP53 was detected in CMTM3 overexpressed JHC7 cells by western blot. F Up, the transfection efficiency of the CMTM3-siRNA was detected in JHC7 cells by western blot. 100 μg of total protein lysates were loaded; Down. the expression of TP53 in CMTM3 silenced JHC7 cells was detected by western blot. Data were representative of three independent experiments