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. Author manuscript; available in PMC: 2022 Sep 16.
Published in final edited form as: Mol Cell. 2021 Sep 16;81(18):3803–3819.e7. doi: 10.1016/j.molcel.2021.08.025

Figure 1. IMPA/Inositol regulates mitochondrial fission.

Figure 1.

(A) Phosphoinositides metabolism with a series of enzymes. (B) Subcellular localization of IMPA1 using IMPA1 antibody (Green), MitoTracker (red) and DAPI (blue) in diverse cancer cell lines. (C and D) Mitochondrial morphology by MitoTracker (red) in shLuc. or shIMPA1 DU145 cells (C) or MEFs (D). Scale bar, 20 μm in MitoTracker and Phase; 5 μm in Enlarge. (E and F) Quantification of the mitochondrial morphology of the DU145 cells (C) and MEFs (D) shown in (E) and (F). (G and H) The shLuc. or shIMPA1 DU145 or MEFs were subjected to immunoblotting. (I) TEM was performed in shLuc. or shIMPA1 DU145 cells of upon vehicle (NT) or inositol treatment (Ins). Scale bar, 500 nm. Blue arrow, healthy/elongated mitochondria; Red arrows, fragmented mitochondria. (J) Quantification of the mitochondrial morphology (long and short) shown in (I).