Key resources table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
IMPA1 (clone H-7)	Santa Cruz Biotechnology	Cat#sc-374234; RRID: AB_10988246
Mff (clone B-2)	Santa Cruz Biotechnology	Cat# sc-398617; RRID: AB_2744543
phospho-Mff Ser146 (polyclone)	Cell Signaling Tech	Cat#49281; RRID: AB_2799354
IMPA2 (polyclone)	GeneTex	Cat#GTX103636; RRID: AB_1950587
AMPKα (polyclone)	Cell Signaling Tech	Cat#2532; RRID: AB_330331
AMPKβ (polyclone)	Cell Signaling Tech	Cat#12063; RRID: AB_ 2797812
AMPKγ (polyclone)	Cell Signaling Tech	Cat#4187; RRID: AB_10695248
phospho-AMPKα T172 (clone D4D6D)	Cell Signaling Tech	Cat#50081; RRID: AB_2799368
PKA (clone A-2)	Santa Cruz, sc-28315	Cat#sc-28315; RRID: AB_628136
phospho-Drp1 S616 (polyclone)	Cell Signaling Tech	Cat#3455; RRID: AB_2085352
phospho-Drp1 S635 (clone D3A4)	Cell Signaling Tech	Cat#6319; RRID: AB_10971640
Fis (clone B-5)	Santa Cruz Biotechnology	Cat#sc-376447; RRID: AB_11149382
Mfn1 (clone D-10)	Santa Cruz Biotechnology	Cat#sc-166644; RRID: AB_2142616
Mfn2 (clone F-5)	Santa Cruz Biotechnology	Cat#sc-515647; RRID: AB_2811176
ULK (clone D8H5)	Cell Signaling Tech	Cat#8054; RRID: AB_11178668
Raptor (clone 24C12)	Cell Signaling Tech	Cat#2280; RRID: AB_561245
phospho-ULK S555 (clone D1H4)	Cell Signaling Tech	Cat#5869; RRID: AB_10707365
phospho-Raptor S792 (polyclone)	Cell Signaling Tech	Cat#2083, RRID: AB_2249475
Actin (clone AC-15)	Sigma-Aldrich	Cat#A5441, RRID: AB_476744
GAPDH (clone GAPDH-71.1)	Sigma-Aldrich	Cat#G8795, RRID: AB_1078991
Tubulin (clone AA13)	Sigma-Aldrich	Cat#T8203, RRID: AB_1841230
CDIPT (clone C-2)	Santa Cruz Biotechnology	Cat#sc-514255
GST (clone B-14)	Santa Cruz Biotechnology	Cat#sc-138, RRID: AB_627677
LC3 α/β (polyclone)	Novus	Cat#NB100-2220, RRID: AB_10003146
Calcineurin (D-1)	Santa Cruz Biotechnology	Cat#sc-373803, RRID: AB_10917925
MiD51 (clone A-6)	Santa Cruz Biotechnology	Cat#sc-514135
CREB (clone 24H2B)	Santa Cruz Biotechnology	Cat#sc-271, RRID: AB_627300
phospho-CREB S133 (clone 10E9)	Santa Cruz Biotechnology	Cat#sc-81486, RRID: AB_1125727
choactase (clone E-7)	Santa Cruz Biotechnology	Cat#sc-55557, RRID: AB_2291743
TOM20 (monoclone)	Abcam	Cat#ab56783, RRID: AB_945896
HRP-coupled antibodies to mouse	Santa Cruz Biotechnology	Cat#sc-2055, RRID: AB_631738
HRP-coupled antibodies to rabbit	Thermo Fisher	Cat#31480, RRID: AB_228457
Alexa Fluor 488 anti-mouse IgG	Thermo Fisher	Cat#A-21202, RRID: AB_141607
Rabbit normal IgG	Santa Cruz Biotechnology	Cat#sc-2027; RRID: AB_737197
Mouse Anti-rabbit IgG	Cell Signaling Tech	Cat#45262; RRID: AB_2799281
Chemicals and recombinant proteins
lithium chloride	Millipore	Cat#5910
AICAR	Sigma-Aldrich	Cat#A9978
A769662	Reagents Direct	Cat#A769662
Compound C	Sigma-Aldrich	Cat#P5499
Myo-inositol	Sigma-Aldrich	Cat#I7508
AMP	Sigma-Aldrich	Cat#A1752
ATP	Sigma-Aldrich	Cat#A2383
BIO	Selleckchem.com	Cat#S7198
L-α-Phosphatidylinositol	Sigma-Aldrich	Cat#P6636
myo-Inositol 1,4,5-trisphosphate hexapotassium salt	Tocris	Cat#1482
phosphatidylinositol 3,4,5-triphosphate	Echelon®	Cat#P-3924
AMPKα1, β1, γ1 protein	Millipore	Cat#14-840
AMPKα1, β1, γ1 protein	Promega	Cat#V1921
AMPKα1, β2, γ1 protein	Thermo Fisher	Cat#PV6244
AMPKα2, β1, γ1 protein	BPS Bioscience	Cat#40024
AMPKα2, β2, γ1 protein	Thermo Fisher	Cat#PV6247
AMPKα1	Novus Biologicals	Cat#NBP2-51992
AMPKβ1	Novus Biologicals	Cat#NBP2-23375
AMPKγ1	Novus Biologicals	Cat#NBP-23377
ULK protein	Thermo Fisher	Cat# PV6430
SAMS peptide	Promega	Cat#V1921
Tetramethylrhodamine	Thermo Fisher	Cat# T669
Dulbecco’s Modified Eagle’s Medium w/o inositol	MP Biomedicals	Cat# 091642954
[3H]AMP	American Radiolabeled Chemicals Inc.	Cat#ART0843
[3H]inositol	American Radiolabeled Chemicals Inc.	Cat#ART0261
Critical commercial assays
K-INOSL Assay Kit	Megazyme	Cat#820517
Glucose-6-Phosphate Colorimetric Assay Kit	BioVision	Cat#K657
ATP Determination Kit	Thermal Fisher	Cat#A22066
ADP-Glo™ kinase assay	Promega	Cat#V6930
Experimental models: Cell lines
Human: PC3	ATCC	Cat#CRL-1435
Human: DU145	ATCC	Cat#HTB-81
Mouse: mouse embryonic fibroblasts	ATCC	Cat#SCRC-1008
Mouse: AMPK−/− mouse embryonic fibroblasts	Dr. Kun-Liang Guan (The University of California, San Diego)	N/A
Experimental models: Organisms/Strains
Mouse: B6SJL.SOD1-G93A	The Jackson Laboratory	JAX: 002726
Mouse: C57BL/6J	The Jackson Laboratory	JAX: 000664
Oligonucleotide
ShRNA: human IMPA1-#1	Sigma-Aldrich	Cat#TRCN0000310192
ShRNA: human IMPA1-#2	Sigma-Aldrich	Cat#TRCN0000296135
ShRNA: mouse IMPA1-#1	Sigma-Aldrich	Cat#TRCN0000081409
ShRNA: mouse IMPA1-#2	Sigma-Aldrich	Cat#TRCN0000306604
ShRNA: human IMPA2-#1	Sigma-Aldrich	Cat#TRCN0000050318
ShRNA: human IMPA2-#2	Sigma-Aldrich	Cat#TRCN0000050320
ShRNA: human CDIPT-#1	Sigma-Aldrich	Cat#TRCN0000035987
ShRNA: human CDIPT-#2	Sigma-Aldrich	Cat#TRCN0000035988
ShRNA: human INPP1-#1	Sigma-Aldrich	Cat#TRCN0000051600
ShRNA: human INPP1-#2	Sigma-Aldrich	Cat#TRCN0000051601
ShRNA: human PI4K2α-#1	Sigma-Aldrich	Cat#TRCN0000195093
ShRNA: human PI4K2α-#2	Sigma-Aldrich	Cat#TRCN0000037605
ShRNA: human PLCβ1-#1	Sigma-Aldrich	Cat#TRCN0000226441
ShRNA: human PLCβ1-#2	Sigma-Aldrich	Cat#TRCN0000226442
ShRNA: human AMPKα1	Sigma-Aldrich	Cat#TRCN0000199831
ShRNA: human AMPKα2	Sigma-Aldrich	Cat#TRCN0000219690
Recombinant DNA
pGEX6P-1-AMPKα	This paper	N/A
pGEX6P-1-AMPKβ	This paper	N/A
pGEX6P-1-AMPKγ	This paper	N/A
pGEX6P-1-Bateman domain 1	This paper	N/A
pGEX6P-1-Bateman domain 2	This paper	N/A
pGEX6P-1-CBS1	This paper	N/A
pGEX6P-1-CBS2	This paper	N/A
pGEX6P-1-CBS3	This paper	N/A
pGEX6P-1-CBS4	This paper	N/A
pFLAG-CMV-2-IMPA1	This paper	N/A
pFLAG-CMV-2-IMPA1 D220A	This paper	N/A
pFLAG-CMV-2-IMPA1 W219A	This paper	N/A
Software and algorithms
ImageJ	ImageJ software	https://imagej-nih-gov.go.libproxy.wakehealth.edu/ij/index.html
Fiji	Fiji software	https://imagej.net/Fiji
Graphpad Prism 8	Graphpad software	N/A
CRISPR Design tool	Zhang Lab	https://zlab.bio/guide-design-resources
Deposited Data
Unprocessed images	This paper	http://dx.doi.org/10.17632/nvf6fgc77g.1