Species-specific nested PCR amplification products for a study of Plasmodium inui infections among humans, Malaysia. Samples were subjected to electrophoresis on a 1.5% agarose gel. A) Results for detection of P. knowlesi, P. falciparum, P. vivax, P. malariae, P. ovale, and P. cynomolgi. Lane 1, human case-patient PMAR0041; lane 2, human case-patient PMAR0052; lane 3, no-template control. B) Results for the detection of P. inui in human case-patient PMAR0041. Lane 1, case-patient PMAR0041; lane 2, negative control; lane 3, no-template control. The solid vertical line indicates these are separate parts of the same image. C) Results for the detection of P. inui in human case-patient PMAR0052. Lane 1, case-patient PMAR0052; lane 2, no-template control.