Figure 1.

Rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) purification of androgen receptor (AR)-interacting proteins in the LNCaP cells. A, AR RIME was performed using 2 distinct anti-AR antibodies (N-20 and H-280). Only proteins identified by both antibodies (195 proteins) were considered, and any protein that was identified in the immunoglobulin G (IgG) control was excluded. B, Two distinct RIME purifications were conducted, and the proteins identified in both were plotted and categorized as chromatin remodelers; C, histone modifiers; and D, known nonenzymatic nuclear receptor (NR) coregulators. The y-axis represents the log10 Mascot score of each replicate. E, Enhanced at puberty 1 (EAP1; IRF2BPL) and its family member IRF2BP2 were identified by RIME and indicated in the scatter plot. F, Total peptide coverage of specific identified proteins. Green highlighting indicates peptides identified with high confidence (false discovery rate < 0.01). G, Schematic of the protein domain structure of IRF2BP family members. All members harbor a zinc finger domain, nuclear localization signal (NLS), and RING finger domain. EAP1 contains only a poly glutamine (Q) repeat motif.