Figure 10.

Angiopoietin/ Tie2 signaling is altered in PIRKO. (A) Angpt1 mRNA expression is reduced in isolated brain pericytes from PIRKO compared with control. (B) and (C) Phosphorylation of Tie2 was assessed in P5 retinal territories (C), and normalized to the mean staining intensity of pTie2 in arteries (B), scale bar 50 µm. (D) pTie2 staining is reduced in the venous plexus PIRKO. (E) P5 retinas were stained against FoxO1 and nuclear FoxO1 localization was assessed by FoxO1/Hoechst colocalization. FoxO1 positive nuclei are labeled with an arrow, scale bar 50 µm. (F) Nuclear FoxO1 in the arterial plexus is similar in both groups, whereas nuclear FoxO1 localization is increased in the venous plexus in PIRKO compared to control. (G) Angpt2 mRNA expression (relative to β-actin) is increased in P5 retinas and (H) P5 lungs in PIRKO compared to control. Data presented as mean ± SEM, unpaired t-test (A, B, F, G, and H), or 2-way ANOVA with Sidak’s multiple comparison test (D), *P < .05, ***P < .001, n = 6, 6 (A), n = 10, 10 (B, D) n = 7, 10 (F) n = 7, 7 (G, H); A, artery; V, vein.