Figure 7.

Assessment of vascular regression and endothelial proliferation. (A) Vessel regression at P5 was assessed by collagen IV empty sleeves, regressed vessels are labeled with an arrow, scale bar 200 µm. (B) Vessel regression is similar between PIRKO and control. (C) Vascular cell proliferation was assessed by EdU-incorporation, scale bar 200 µm. (D) There is no difference in vascular cell proliferation between the groups. Data presented as mean ± SEM, unpaired t-test, not significant, n = 13,13 (B), n = 8,8 (D); A, artery; V, vein.