Figure 8.

Retinal arteriovenous commitment marker expression. Arterial- and venous commitment markers (A) DLL4, (B) EphB4, and (C) Coup-TFII were qualitatively assessed in P5 retina and appear to be adequately expressed at the vascular plexus and vascular front, scale bar 50 µm. (D) mRNA expression of Notch target genes Hes1, Hey1, Nrarp are unchanged in PIRKO in P5 retinas. Data (D) are presented as mean ± SEM, relative expression corrected for β-actin, unpaired t-test not significant, n = 6,9 (A-C), n = 7,5 (D); A, artery; V, vein.