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. 2021 Sep 24;41(10):e00609-20. doi: 10.1128/MCB.00609-20

FIG 2.

FIG 2

Influence of circ-ERBB2 inhibition on the Warburg effect and malignancy of TNBC cells. (A) The relative expression of circ-ERBB2 in MDA-MB-231 and MDA-MB-468 cells transfected with si-circ-ERBB2#1, si-circ-ERBB2#2, or si-circ-ERBB2#3 was validated by qRT-PCR. si-NC acted as a control. (B to H) The proliferation, apoptosis, migration, and invasion of MDA-MB-231 and MDA-MB-468 cells transfected with si-circ-ERBB2#1, si-circ-ERBB2#2, or si-NC were assessed by a colony formation assay, MTT assay, flow cytometry assay, wound-healing assay, or transwell assay. (I to L) An XF96 glycolysis analyzer was utilized to analyze the ECAR and OCR in MDA-MB-231 and MDA-MB-468 cells transfected with si-circ-ERBB2#1, si-circ-ERBB2#2, or si-NC. (M and N) The levels of glucose consumption and lactate production in MDA-MB-231 and MDA-MB-468 cells transfected with si-circ-ERBB2#1, si-circ-ERBB2#2, or si-NC were detected with matching kits. 2-DG, 2-deoxy-d-glucose; PI, propidium iodide; mPH: miles per hour; FCCP, carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone. In addition, PE-A in panel E comes with the apoptosis detection instrument. *, P < 0.05.