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. 2021 Sep 24;41(10):e00609-20. doi: 10.1128/MCB.00609-20

FIG 6.

FIG 6

miR-136-5p decreased the Warburg effect and repressed the malignancy of TNBC cells via targeting PDK4. (A) Western blotting revealed the protein level of PDK4 in MDA-MB-231 and MDA-MB-468 cells transfected with pcDNA or PDK4. (B to N) MDA-MB-231 and MDA-MB-468 cells were transfected with NC mimics, miR-136-5p mimics, miR-136-5p mimics plus pcDNA, or miR-136-5p mimics plus PDK4. (B) The protein level of PDK4 in MDA-MB-231 and MDA-MB-468 cells was surveyed using Western blotting. (C to H) The proliferation, apoptosis, migration, and invasion of MDA-MB-231 and MDA-MB-468 cells were evaluated using a colony formation assay, MTT assay, flow cytometry assay, wound-healing assay, or transwell assay. (I to L) The ECAR and OCR of MDA-MB-231 and MDA-MB-468 cells were measured by an XF96 glycolysis analyzer. (M and N) Glucose consumption and lactate production in MDA-MB-231 and MDA-MB-468 cells were determined using the corresponding kits. *, P < 0.05.