TABLE 2.
Target | Direction | Primer sequence |
---|---|---|
circ-ERBB2 | F | 5′-ACGTTTGAGTCCATGCCCAA-3′ |
R | 5′-ACCTGCCTCACTTGGTTGTG-3′ | |
ERBB2 | F | 5′-GGAAGTACACGATGCGGAGACT-3′ |
R | 5′-ACCTTCCTCAGCTCCGTCTCTT-3′ | |
PDK4 | F | 5′-AGGTGGAGCATTTCTCGCGCTA-3′ |
R | 5′-GAATGTTGGCGAGTCTCACAGG-3′ | |
miR-1276 | F | 5′-GCGCGTAAAGAGCCCTGTG-3′ |
R | 5′-AGTGCAGGGTCCGAGGTATT-3′ | |
miR-136-5p | F | 5′-CGCGACTCCATTTGTTTTGAT-3′ |
R | 5′-AGTGCAGGGTCCGAGGTATT-3′ | |
miR-503-5p | F | 5′-CGTAGCAGCGGGAACAGTT-3′ |
R | 5′-CGTAGCAGCGGGAACAGTT-3′ | |
miR-520f-3p | F | 5′-CGCGAAGTGCTTCCTTTTAG-3′ |
R | 5′-CGCGAAGTGCTTCCTTTTAG-3′ | |
U6 | F | 5′-CGCTTCGGCAGCACATATAC-3′ |
R | 5′-TTCACGAATTTGCGTGTCATC-3′ | |
β-Actin | F | 5′-GTCACCAACTGGGACGACAT-3′ |
R | 5′-GAGGCGTACAGGGATAGCAC-3′ | |
GAPDH | F | 5′-TGACTTCAACAGCGACACCCA-3′ |
R | 5′-CACCCTGTTGCTGTAGCCAAA-3′ |
F, forward; R, reverse; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.