FIG 2.

Impaired skin permeability barrier formation in Aldh3a2 Aldh3b2 DKO mice. (A and B) Aldh3b2 KO and DKO mice were photographed on postnatal day 0 (P0) (A) and subjected to scanning electron microscopy (B). Bars, 100 μm. (C and D) Time courses of survival rates (C) and body weights (D) of Aldh3b2 KO (n = 4) and DKO (n = 8) mice after Caesarean section, examined at embryonic day 18.5 (E18.5). Values presented in panel D are means ± SD, and statistically significant differences from Aldh3b2 KO mice are indicated (*, P < 0.05; **, P < 0.01; Student’s t test). (E) Transepidermal water loss (TEWL) from WT (n = 8), Aldh3b2 KO (n = 13), and DKO mice (n = 16) were measured at P0. Values presented are means ± SD, and statistically significant differences are indicated (**, P < 0.01; Tukey’s test). (F) Aldh3b2 KO and DKO mice at P0 were stained with 0.1% toluidine blue for 40 h and photographed. b2 KO, Aldh3b2 KO.