FIG 8.

Expression levels of acylceramide synthesis-related genes and keratinocyte differentiation markers. (A and B) Total RNA prepared from the epidermis of WT (n = 3), Aldh3b2 KO (n = 3), and Aldh3a2 Aldh3b2 DKO (n = 3) mice at P0 was subjected to SYBR green-based quantitative real-time RT-PCR using specific primers for acylceramide synthesis-related genes (Cyp4f39, Fatp4, Cers3, Pnpla1, and Abhd5) (A) and keratinocyte differentiation markers (Krt1, Krt5, Lor, and Ivl) (B). Values are expressed relative to Hprt1 and are means ± SD. b2 KO, Aldh3b2 KO.