FIG 6.

HNRNPU-AS1 silencing promoted cell proliferation and inhibited apoptosis by promoting the Wnt/β-catenin signaling pathway via the miR-205-5p/AXIN2 axis. When siHNRNPU-AS1 or miR-205-5p mimic was cotransfected with ANXI2, CCK-8 assay was conducted for cell viability (A and B), colony formation assays for cell proliferation (C and D), flow cytometric assay for cell apoptosis (E and F), RT-qPCR and WB assay for gene levels (G and H), and WB assay for DDK1 and β-catenin expression (I and J). Data are displayed in the form of means ± SD from 3 individual assays. **, P < 0.01 versus siNC+mimic NC group; ^^, P < 0.01 versus ANXI2 group; ##, P < 0.01 versus mimic group.