FIG 7.

HNRNPU-AS1 overexpression inhibited cell proliferation and promoted apoptosis by promoting the Wnt/β-catenin signaling pathway via the miR-205-5p/AXIN2 axis. After cotransfection with miR-205-5p inhibitors or vector expressing HNRNPU-AS1 and siANXI2, CCK-8 assay was conducted for cell viability (A and B), colony formation assay for cell proliferation (C and D), flow cytometric analysis for cell apoptosis (E and F), RT-qPCR and WB assays for Bcl-2, Ki67, and Bax expression (G and H), and WB assay for β-catenin and DDK1 expression (I and J). Data were displayed in the form of means ± SD from 3 individual assays. **, P < 0.01 versus siNC+inhbitor NC group; ^^, P < 0.01 versus siANXI2 group; ##, P < 0.01 versus inhibitor group.