KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal to Orai1	Sigma-Aldrich	Cat#O8264; RRID:AB_1078883
Chemicals, peptides, and recombinant proteins
2-aminoethoxydiphenyl borate	Millipore-Sigma	Cat#8.41636
Fura 2-acetoxymethyl ester	Sigma-Aldrich	Cat#F0888
Ionomycin	Sigma-Aldrich	Cat#I9657
EGTA	Sigma-Aldrich	Cat#324626
YFP-SOAR concatemers	Zhou et al., 2015 (Mutagenix)	N/A
NFAT1-GFP	Yoast et al., 2020	N/A
Poly-L-Lysine	Sigma-Aldrich	Cat#P4832
Thapsigargin	Sigma-Aldrich	Cat#T9033
Carbachol	Sigma-Aldrich	Cat#212385-M
Critical commercial assays
QuikChange Lightning Site-Directed Mutagenesis Kit	Agilent	Cat#210518
Experimental models: Cell lines
HEK293 cells	ATCC	Cat#CRL-1573; RRID:CVCL_0045
HEK293 Orai1-3 TKO cells	Yoast et al., 2020	N/A
HEK293 STIM1/2 DKO cells	Zhou et al., 2018a	N/A
HeLa-GFP-NFAT1 cells	Zhou et al., 2016	N/A
Jurkat T cells	ATCC	Cat#TIB-152; RRID:CVCL_0367
Oligonucleotides
O1-M4x Forward: GGCATGGACGAGCTGTACAAGAAGCTTGCTGACCGACAGTTCCAGGAGCTCA	Zhou et al., 2015	N/A
O1-M4x Reverse: ACAGCTCATCCTTAAGTCGACTCGAGATGCGCTAGGCAsTAGTGGCTGCCGGG	Zhou et al., 2015	N/A
O2-M4x Forward: GGCATGGACGAGCTGTACAAGAAGCTTGCTGAGCGCCACAACCGCGAGATCG	This paper	N/A
O2-M4x Reverse: GGCCCGCGGTACCGTCGACTGCAGAATTCGCCTACAAGACCTGCAGGCTGCG	This paper	N/A
O3-M4x Forward: GGCATGGACGAGCTGTACAAGAAGCTTGCTGACCGCTACAAGCAGGAACTAG	This paper	N/A
O3-M4x Reverse: GGCCCGCGGTACCGTCGACTGCAGAATTCGCTCACACAGCCTGCAGCTCCCC	This paper	N/A
O1-O3 M4x mutant primers	This paper	See Table S1
Software and algorithms
SlideBook Software Ver. 6.0	Intelligent Imaging Innovations	https://www.intelligent-imaging.com
GraphPad Prism 6, 8, and 9	GraphPad Software, Inc.	https://www.graphpad.com
Excel	Microsoft	https://www.microsoft.com
WebMaxCLITE	Stanford	http://web.stanford.edu/Bcpatton/webmaxc2
SnapGene Viewer	SnapGene	https://www.snapgene.com
DNASTAR Lasergene Software	DNASTAR	https://www.dnastar.com
Other
FluorChem M Imaging System	Protein-simple	N/A