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Cloned DNA fragments containing the ALK KD region (ALK exons 20 to 28 (4080–5020; NM_004304.3), serially diluted in genomic DNA from HL-60 cells). The arrows indicate the ALK primer of the ALK KD region. PCR was conducted to determine analytical sensitivity; plasmid from ALK KD and HL-60 was mixed in ratios ranging from 105 to 100 copies. The analytical sensitivity of ALK KD screening was estimated using one copy of mutations in a normal background.
