Figure 7.

Myo-inositol impacts the TCA cycle and promote tilapia’s ability against bacterial infection. (A) Survival of tilapias post A sobria infection in the presence of myo-inositol. Tilapias were treated with saline control (0 µg per fish) or different doses of myo-inositol (200, 400, and 800 µg per fish) at 33°C for 3 days, followed by bacterial challenge through intraperitoneal injection (1 × 10⁵ CFU A. sobria). The accumulative fish death was monitored for a total of 15 days postinfection (n =30 per group). (B) Activity of PDH, KGDH, SDH, and MDH of spleens at 18°C and 33°C. (C) Activity of PDH, KGDH, SDH, and MDH of spleens in the presence or absence of 800 µg myo-inositol per fish. Values are means ± SEM (n = 6 per group), and statistic difference is analyzed with non-parametric Kruskal–Wallis one-way analysis with Dunn’s multiple-comparison post hoc test. *p < 0.05; **p < 0.01 (B, C).