Figure 8.

Innate immune responses of tilapias. (A) qRT-PCR for innate immune genes of tilapia treated with saline solution (control) or 800 µg myo-inositol for 3 days following A. sobria challenge through intraperitoneal injection (1 × 10³ CFU A. sobria). Spleens were collected at 0, 3, 6, and 9 h postinjection for RNA extraction and qRT-PCR. Values are means ± SEM from six biological replicates. **p < 0.01. (B) Survival of tilapias post A. sobria infection in the presence of myo-inositol. Tilapias were treated with saline control (0 µg per fish) or different doses of myo-inositol (200, 400, and 800 µg per fish) at 18°C for 3 days, followed by bacterial challenge through intraperitoneal injection (1 × 10⁵ CFU A. sobria). The accumulative fish death was monitored for a total of 15 days postinfection (n =30 per group).