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. 2021 Sep 14;17(9):e1009807. doi: 10.1371/journal.pgen.1009807

Fig 1. Chd1 recruitment to a DSB and histone removal.

Fig 1

(A) Exponentially growing YEPR cell cultures of JKM139 derivative strains were transferred to YEPRG, followed by Chd1-Myc ChIP at the indicated distances from the HO-cut site compared to untagged Chd1 (no tag). Data are expressed as fold enrichment at the HO-cut site over that at a non-cleavable locus (ARO1), after normalization to the corresponding input for each time point. Fold enrichment was then normalized to cut efficiency. Plotted values are the mean values ± s.d. from three independent experiments. ***P<0.005, **P<0.01, *P<0.05, t-test. (B) HO expression was induced by galactose addition to G2-arrested cells carrying the HO system at the LEU2 or at the MAT locus. Cells were kept arrested in G2 by nocodazole throughout the experiment. Histone H3 ChIP with anti-H3 antibody at the indicated distances from the HO-cut site. Data are expressed as fold enrichment at the HO-cut site over that at the non-cleavable ARO1 locus after normalization to the corresponding input for each time point. Fold enrichment was normalized to cut efficiency. Plotted values are the mean values ± s.d. from three independent experiments.