Figure 4.

Induction of oxidative burst by monocytes from patients with DL, CL or healthy subjects following infection with different L. braziliensis isolates: PBMC from patients with DL (n=12), CL (n=12) or HS (n=8) were treated with DHR (10ng/mL; 10 min) and infected with L. braziliensis isolates from DL or CL patients for 25 minutes at a ratio of 5:1 cells. PMA (1ug/ml) was used as positive control. Cells were stained with anti-CD14 for flow cytometric evaluation. (A) Representative gating strategy detailing CD14+ and DHR expression in monocytes from a CL patient. (B) Data representative as median of the mean fluorescence intensity (MFI) of oxidative burst induction in DL and CL monocytes infected with different isolates. All p values were obtained using Wilcoxon’s signed-rank testing. Comparisons made between groups using Mann- Whitney statistical testing. (*p < 0.05).