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. 2021 Jun 9;33(9):3134–3150. doi: 10.1093/plcell/koab161

Figure 1.

Figure 1

Expression of PLL12 affects stomatal function. A, Stomatal responses to 50 µM ABA in Col (black), pll12-1 (magenta), and PLL12OE (green) genotypes. Error bars indicate standard error of the mean (sem); n ≥ 121 stomata per genotype per time point from three independent experiments. Different letters at each timepoint indicate P < 0.05 across genotypes for that timepoint, one-way analysis of variance (ANOVA) and Tukey’s test (dashed gray box indicates statistical comparison group). B, Representative images of stomata in Col, pll12-1, and PLL12OE plants before and after 2.5 h ABA treatment. Stomatal complexes and pores are outlined by yellow dashed lines. Bar = 5 µm. C–D, As in (A–B) but treated with 1 µM FC and n ≥ 122 stomata per genotype per timepoint. Bar = 5 µm. E, Comparison of stomatal responses to 50 µM ABA between GFP kd-2 (black) and PLL12 kd-2 (blue) plants. Error bars indicate SEM; asterisks indicate significant difference between genotypes at each time point examined: ****P < 0.0001, Student’s t test; n ≥ 102 stomata per genotype per time point from three independent experiments and six biological replicates (individual leaves from different plants) in total. F, As in (B) but for GFP kd-2 and PLL12 kd-2. Images without outlines of PLL12 kd2 are also shown. Bar = 5 µm. G–H, As in (E–F) but treated with 1 µM FC and n ≥ 112 stomata. Bar = 5 µm.