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. Author manuscript; available in PMC: 2021 Nov 18.
Published in final edited form as: J Am Chem Soc. 2020 Nov 3;142(46):19493–19501. doi: 10.1021/jacs.9b12426

Figure 2.

Figure 2.

Photoactivation of HA-Cys-Ub-TZ probe and labeling of deubiquitinating enzyme: (A) ESI-MS characterization of HA-Cys-Ub-TZ probe with 365 nm UV treatment for 15 min; (B) ESI-MS characterization of USP2-CD incubated with HA-Cys-Ub-TZ under 365 nm UV treatment for 15 min; (C) proposed reaction mechanism of HA-Cys-Ub-TZ with deubiquitinating enzyme USP2-CD under 365 nm UV treatment.