Fig. 5.

(a) Agarose gel electrophoresis result of the mTS-mPAE nanoparticles. NPs formed from polymer to siRNA at w/w ratio of 1:1, 10:1, 25:1, 100:1, 200:1 and 300:1. Samples were prepared at room temperature before being loaded onto a 1.0% w/v agarose gel (75 V, 45 min). (b) Densitometric analysis of the siRNA bands performed using Image J software. The experiments were repeated at least thrice. The best representative images are shown.