Fig. 9. Silencing efficiency of MS-DF (mTOR-siRNA Dharmafect transfection reagent complex) transfection reagent and mTS-mPAE NPs at 50 nM concentration.
Expression of mTOR protein determined using Western blot in A549 and H460 cells (2× 105 cell/well). mTS-MPAE NPs/mTOR siRNA and SS-MP NPS/scrambled siRNA prepared at weight ratio 45:1 (siRNA concentration 50 nM or 1.33μg per well) (a) Western blot showed a marked reduction in the mTOR protein levels after treatment with mTS-mPAE NPS at 50 nM concentrations in A549 cells. (c) Western blot showed a marked reduction in the mTOR protein levels after treatment with mTS-mPAE NPS at 50 nM concentrations in H460 cells. (b) mTOR protein silencing was calculated using the densitometric analysis and β-Actin as loading control in A549 cells and (d) mTOR protein silencing was calculated using the densitometric analysis and β-Actin as loading control in H460 cells. The results are presented as mean ± SD (n = 3). (* p < 0.05, ** p < 0.01).