Table 3. Kinetic parameters from nucleosome sliding assays.

Rates are expressed as the mean ± standard deviation from three independent measurements (NA: not applicable).

Condition	Sliding rate (a. u.) at [ALC1] = 31.25 nM	[ALC1] at peak (nM)	Sliding rate at peak (a.u.)	Sliding rate at saturation ([ALC1] = 2 μM)
-HPF1	0.059 ± 0.002	NA	NA	0.101 ± 0.015
+ HPF1	0.144 ± 0.009	31.25	0.144 ± 0.009	0.102 ± 0.016
+ HPF1, ALC1fl R611A, S612A	0.085 ± 0.025	62.50	0.110 ± 0.003	0.068 ± 0.014
+ HPF1, APM nuc	0.029 ± 0.009	250.00	0.075 ± 0.003	0.032 ± 0.009
+ HPF1, gH4 nuc	0.038 ± 0.004	125.00	0.091 ± 0.010	0.022 ± 0.002