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Phosphorylation of STAT3, AKT, and S6 in hearts of diabetic WT and STAT3-deficient mice following I/R injury. High-fat-diet (HFD)-fed WT and STAT3-deficient mice were treated with RAPA for 28 days. (A) Representative immunoblots of phospho-STAT3, STAT3, Phospho-AKT, AKT, Phospho-S6, S6 and GAPDH in hearts of WT, and STAT3-deficient mice following I/R injury. (B–G) Densitometry analysis of the ratios of phosphorylated (p) to total protein, total protein to GAPDH (n = 3; one-way ANOVA).
