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. 2020 Oct 24;35(11):2454–2466. doi: 10.1093/humrep/deaa223

Figure 3.

Figure 3.

Abnormal IL-27 and Gal-9 expression in recurrent pregnancy loss (RPL) patients. (A, C) Expression levels of Gal-9 and IL-27 mRNA in placental villi from early pregnancy in patients who have suffered RPL (n = 16) and patients carrying normal pregnancies (NP) (n = 28). (B, D) Protein levels of Gal-9 (40kDa) and IL-27 (27kDa) in early pregnancy villi from RPL (>2 unexplained spontaneous abortions), miscarriage (a single unexplained spontaneous abortion) and normal pregnancy were determined by western blotting normalized to levels of β-actin (42 kDa). Relative band intensities were quantified using ImageJ. Results are shown as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.