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. 2021 Sep 17;2021:6633886. doi: 10.1155/2021/6633886

Figure 1.

Figure 1

The effects of raw or roasted Schisandra chinensis (Turcz.) Baill (SC), Lycium chinense Mill. (LC), or mass-based 1 : 1 mixture, SL, on LPS-stimulated nitric oxide (NO) production, ROS generation, and the protein expression of iNOX, COX-2, and NADPH oxidases (NOXs) in RAW264.7 macrophages. RAW264.7 cells were pretreated with raw or roasted (R) SC, LC, or SL (500 μg/mL) for 1 h and then incubated with LPS (1 μg/mL) for 18 h. (a–e) The culture media were collected for NO production, cell lysates were prepared for ROS generation, and iNOS, COX-2, NOX (−1, −2, and −4), and p22phox, p47phox, and p67phox levels were analyzed by immunoblotting. The densitometry data represented are shown as the relative density of protein bands normalized to β-actin level.  ∗∗∗P < 0.001 vs. blank B; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. control (C: LPS treatment group). ǂP < 0.05 vs. RSC, RLC, and SL. Values are the mean ± SEM of three independent experiments.