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Conditions of chromatography for purifications.
| Time (min) | 0.1% TFA in ddwater (%) | 0.1% TFA in acetonitrile (%) | Flow (mL/min) |
|---|---|---|---|
| 0 | 70 | 30 | 3.0 |
| 6 | 70 | 30 | 3.0 |
| 25 | 45.3 | 54.7 | 3.0 |
| 26 | 5 | 95 | 3.0 |
| 28 | 5 | 95 | 3.0 |
| 29 | 70 | 30 | 3.0 |
Fractions were collected then lyophilized.
